Bacteriological Profile and Antimicrobial Susceptibility Pattern among Isolates Obtained From Body Fluids

  • Lok Bahadur Shrestha Department of Microbiology and Infectious Diseases, B. P. Koirala Institute of Health Sciences, Dharan, Sunsari, Nepal
  • Narayan Raj Bhattarai Department of Microbiology and Infectious Diseases, B. P. Koirala Institute of Health Sciences, Dharan, Sunsari, Nepal
  • Basudha Khanal Department of Microbiology and Infectious Diseases, B. P. Koirala Institute of Health Sciences, Dharan, Sunsari, Nepal

Abstract

Background: Infections of the sterile body sites, when occurs typically have greater clinical urgency and these infections could be life threatening. For the appropriate management of patient, early detection and identification of organism is crucial. Hence, the present study was designed to evaluate the prevalence, organism profile and antimicrobial susceptibility pattern of isolates obtained from infection of body fluids.
Methods: Laboratory and clinical records of all the sterile body fluid except blood and cerebrospinal fluid submitted to the Department of Microbiology, B. P. Koirala Institute of Health Sciences during the period of 5 years (January 2012 to December 2016) were analyzed.
Results: Among 1835 samples, 196 fluids samples showed growth of organisms with an isolation rate of 10.68%. Escherichia coli (E. coli) was the most common organism followed by Staphylococcus aureus (S. aureus) and Acinetobacter calcoaceticus-baumannii complex. Antimicrobial susceptibility testing showed variable degree of resistance. Thirty percentage of organisms were multi-drug resistant, 10% were extensively-drug resistant, none was pan-drug resistant, 35% of E. coli and K. pneumoniae were extended spectrum ?-lactamase producer and 30% of S. aureus were methicillin-resistant S. aureus. The study showed increasing trends of multi-drug resistant, extensively-drug resistant, methicillin-resistant S. aureus and extended spectrum ?-lactamase over the years.
Conclusions: The study showed infection rate of 10% among normally sterile body fluids. E.coli, S. aureus and Acinetobacter calcoaceticus-baumannii complex are the common organisms. The infections are associated with multi drug resistant organisms. Routine surveillance of multi-drug resistant in infection of body fluids is necessary to guide treatment.
Keywords: Extensively-drug resistant; methicillin-resistant S. aureus; multi-drug resistant; sterile body fluid.

Published
2019-08-04
How to Cite
SHRESTHA, Lok Bahadur; BHATTARAI, Narayan Raj; KHANAL, Basudha. Bacteriological Profile and Antimicrobial Susceptibility Pattern among Isolates Obtained From Body Fluids. Journal of Nepal Health Research Council, [S.l.], v. 17, n. 2, p. 173-177, aug. 2019. ISSN 1999-6217. Available at: <http://jnhrc.com.np/index.php/jnhrc/article/view/1656>. Date accessed: 19 oct. 2019. doi: https://doi.org/10.33314/jnhrc.v0i0.1656.