Surgical Site Infections and Antimicrobial Resistance Pattern

  • Raskin Chaudhary Department of Microbiology, St. Xavier’s College, Kathmandu, Nepal.
  • Shrawan Kumar Thapa Orthopaedic Department, Bharatpur Hospital, Chitwan, Nepal.
  • Jid Chani Rana Laboratory Department, Bharatpur Hospital, Chitwan, Nepal.
  • Pradeep Kumar Shah Department of Microbiology, Tri- Chandra College, Kathmandu, Nepal.


Background: Post-operative surgical site infections (SSIs) are among the leading cause of morbidity and increased medical expense. The aim of this study is to isolate identify and study antimicrobial susceptibility pattern of microorganism from surgical wound of admitted patients.
Methods: This retrospective study was carried at the Microbiology Laboratory of Bharatpur hospital, Nepal, from May 2015 to October 2015. The pus samples were cultured and antibiotic susceptibility determined in vitro by Kirby Bauer’s disc diffusion method following clinical and Laboratory Standards Institute (CLSI) 2014 recommendation.
Results: Of the total 250 samples, 194 (77.6%) showed bacterial growth. Staphylococcus aureus was 47.4% and Escherichia coli 20.60 %. Of 194 isolates 39.2% were multi drug resistant. Amikacin was sensitive in 93.1% of Gram positive isolates and 81.8% of gram negative isolates.
Conclusions: Bacterial growth is common in surgical site. Staphylococcus aureus and Escherichia coli were multidrug resistant. Grampositive and gram negative isolates were commonly sensitive to Amikacin.
Keywords: Antibiotic susceptibility; multidrug resistant bacteria; surgical site infections; Staphylococcus aureus.

How to Cite
CHAUDHARY, Raskin et al. Surgical Site Infections and Antimicrobial Resistance Pattern. Journal of Nepal Health Research Council, [S.l.], v. 15, n. 2, p. 120-123, sep. 2017. ISSN 1999-6217. Available at: <>. Date accessed: 06 june 2020. doi: